甘油酯的液相色谱分析

Determination of Glycerides by HPLC

摘要:

对比了反相高效液相色谱－蒸发光散射检测器（RP-HPLC-ELSD）和正相高效液相色谱－示差检测器（NP-HPLC-RI）两种检测方法对甘油醇解大豆油反应产物的检测，结果显示两种方法均能定量分析产物中甘油一酯（MAG）、甘油二酯（DAG）及甘油三酯（TAG）的相对含量；采用了反相高效液相色谱－紫外检测器（RP-HPLC-UV）检测油酸甘油酯，并分别建立了1-油酸甘油一酯（1-O）、1,3-油酸甘油二酯（1,3-OO）和油酸甘油三酯（OOO）的标准曲线图，各标准曲线的相关系数均大于0.99。

Abstract:

In this paper, reverse phase HPLC-ELSD and normal phase HPLC-RI were used to determine the products from glycerolysis of soybean oil. The results indicated that the two methods were suitable for determination of the relative content of monoacylglycerols (MAG), diacylglycerols (DAG) and triacylglycerols (TAG). Oleic acid glycerides were determined by reverse phase HPLC-UV, the standard curves of 1-monoolein (1-O), 1,3-diolein (1,3-OO) and triolein (OOO) were established, and the correlation coefficients were higher than 0.99.