[关键词]
[摘要]
通过单因素、正交实验及其方差分析探索超声波辅助提取甜玉米芯多酚的最优条件。采用Folin-Ciocalteu法测定提取液的总酚浓度。结果表明,各种因素对提取率影响的顺序为:其中对总酚的提取有显著影响。最佳提取工艺为:固液比1:15 (g/mL),乙醇浓度80%,提取温度40 ℃,超声功率200 W,提取时间45 min,在最佳工艺条件下多酚提取率达(2.61±0.09)%,提取液DPPH自由基清除率为(70.05±0.17)%。
[Key word]
[Abstract]
Single factor and orthogonal array design experiments were used to optimize the ultrasonic extraction conditions of polyphenol from sweet corncobs. The total polyphenol content was determined by the Folin-Ciocalteu assay. Results showed that the optimum extraction conditions were as follows: 45 min ultrasonic treatment at 200W power for extracting the raw material with a 15-fold volume of 80% aqueous ethanol solution at 40 ℃. The yield of polyphenol was (2.61±0.09)%, under the optimum condition, and DPPH radical scavenging activity under this condition reached (70.05±0.17)%.
[中图分类号]
[基金项目]
中央高校基本科研业务费资助项目(2011ZB0012)