色谱法监测β-D-吡喃葡萄糖苷的合成

Chromatography Technology for Monitoring the Synthesis of β-D-Glucopyranosides

摘要:

用改进的Koenigs-knorr法立体选择性地合成了2,3,4,6-四乙酰基-香叶基-1-O-β-D-吡喃葡萄糖苷(GLY-A)和香叶基-β-D-吡喃葡萄糖苷(GLY-B)两种键合态香料前体。采用薄层色谱法(TLC)监控反应条件；硅胶柱层析色谱法(SGCC)分离并纯化产物；反向液相色谱法(RP-HPLC)测定样品纯度，得到GLY-A、GLY-B的纯度分别为98.2%、96.0%。色谱技术分别从反应进程的监控、产物的分离纯化、目标产物的纯度检测三个方面，综合应用于糖苷的立体选择性合成中，得到较好的实验结果。

Abstract:

Two glycosidically bond flavor precursors 2,3,4,6-tetraacetyl-geranyl-1-O-β-D-glucopyranoside (GLY-A) and geranyl-β-D-glucopyranoside (GLY-B) were stereo-synthesized by the modified Koenigs-Knorr reaction. Thin layer chromatography (TLC) was used to monitor the reaction. Silica gel column chromatography was used for separating and purifying the samples.By reverse phase HPLC (RP-HPLC), the purity of GLY-A and GLY-B were analyzed as 98.2% and 96.0%, respectively. The chromatography technology was suitable for the monitoring of the stereo-synthesis of glycosides in monitoring, separation and analysis, obtained an ideal experimental results.