尖紫蛤全脏器中糖胺聚糖提取工艺的研究

Extraction of Glycosaminoglycan from the Whole Viscera of Sanguinolaria acut

摘要:

采用单因素实验，研究尖紫蛤全脏器中糖胺聚糖的分离提取工艺条件并进行初步纯化,同时进行电泳鉴定。尖紫蛤全脏器中糖胺聚糖的最佳分离提取条件为：木瓜蛋白酶和枯草杆菌中性蛋白酶用量分别为0.6%和0.79%,水料比为1:1, 酶解温度为58 ℃,酶解时间为4 h，经酶解、离心去蛋白、醇沉、洗涤、干燥，得糖胺聚糖粗制品(SAG-0)，得率为2.58%,糖胺聚糖含量为26.7%。粗制品经脱色、等电点沉淀、超滤、醇沉、洗涤、干燥，得糖胺聚糖精制品(SAG-1)，得率为1.48%,糖胺聚糖含量为40.1%。

Abstract:

The extraction of glycosaminoglycan from the whole viscera of the Sanguinolaria acuta was studied by single factor experiment. And the extracts was preliminarily purified and identified by electrophoresis. The optimal conditions for the enzymatic extraction of glycosaminoglycan from Sanguinolaria acuta were determined as follows: papain dosage 0.6%, subtilisin dosage 0.79%, the ratio of purified water to material 1.0:6, enzymolysis temperature 58 ℃, and enzymolysis time 4 h. Crude glycosaminoghycan (SAG-0) was achieved with the yield of 2.58%. After enzymatic extraction, deproteinization by centrifugation, ethanol precipitation, washing and drying, SAG-0 was achieved with the glycosaminoglycan content of 26.7%. Then SAG-0 was purified by decoloration, isoelectric precipitation, hyperfiltration, ethylalcohol precipitation, washing and drying, giving glycosaminoglycan superfine products (SAG-1) with the yield rate and glycosaminoglycan content being of 1.48% and 40.1%, respectively.