黄斑明目胶囊对MNU诱导大鼠视网膜光感受器细胞损伤的防护作用

Protective Effects of Huangbanmingmu Capsule on Retinal Photoreceptor Damage of Rats Induced by MNU

摘要:

本文探讨了黄斑明目胶囊（含桑椹、菊花、酵母、胡萝卜素、党参、白术、苦茯苓、薏苡仁、丹参，中山眼科中心制）对MNU诱导的大鼠视网膜光感受器细胞变性动物模型的防护作用。将鼠龄为43 d的♀性SD大鼠33只随机分为3组。黄斑明目胶囊组（n=10）以黄斑明目胶囊溶解后灌胃，正常对照组（n=10）和模型对照组（n=10）以等量蒸馏水灌胃，bid，连续7 d。给药7 d后，黄斑明目胶囊组和模型对照组大鼠予MNU 40 mg·kg-1，ip；正常对照组注射等量生理盐水。观察MNU处理后12、24、48、72 h各组大鼠视网膜电图a波及b波的变化，并于MNU作用后7d处死大鼠，取眼球测定视网膜全层及外核层厚度。结果表明正常对照组大鼠不同时间点视网膜电图（ERG）a波及b波的振幅差异无统计学意义（P＞0.05）；模型对照组在MNU处理后ERG a波及b波的振幅均呈持续性、进行性下降，黄斑明目胶囊可明显抑制ERG a波及b波振幅的降低(P＜0.05)。眼病理结果，黄斑明目胶囊组与模型对照组对照组比较，MNU诱导的大鼠视网膜光感受器细胞损伤明显减轻(P＜0.05)。

Abstract:

To approach the protective effects of Huangbanmingmu capsule on retinal photoreceptor damage of rats induced by N-Methyl-N-nitrosourea (MNU), 43-day-old female SD rats were randomly divided into control group, model control group and Huangbanmingmu Capsule group. Huangbanmingmu capsule was intragastricly administrated into rats of Capsule group twice one day for continuous 7 days. For Control group and model group, equal water was used. SD rats in 7 days after treatment received a single injection of 40 mg·kg-1 MNU in model group and Huangbanmingmu Cap sule group, ip; while control group was given equal normal saline. Change of a wave and b wave of electroretinogram (ERG) were observed in 12, 24, 48, 72 hours after MNU injection. The rats were killed in 7 days after MNU treatment. Retinal full-thickness and outer nuclear layer thickness were determined. Results showed that the difference of the average amplitude of ERG a-wave and b-wave was not significant at different time in control group( P＞0.05). And the average amplitude of ERG a-wave and b-wave gradually decreased after MNU treatment in model group. Huangbanmingmu capsule could evidently inhibit lose of a-wave and b-wave, P＜0.05. Ocular pathology showed that the retinal photoreceptor damage of rats induced by MNU was obviously relieved in Huangbanmingmu Capsule group, compared with model control group (P＜0.05）.