响应面法优化葛根多糖提取工艺的研究

Optimization of the Extraction Technology of Polysaccharides from Pueraria by Response Surface Analysis

摘要:

本试验以野葛葛根提取类黄酮后的残渣为材料，研究了葛根多糖的提取工艺。在单因素实验的基础上，选定温度、时间和料液比三个因素的三个水平进行中心组合实验，建立多糖得率的二次回归方程，通过响应面分析及岭嵴分析得到优化组合条件。研究结果表明：当提取工艺条件为提取温度98 ℃，提取时间2.4 h，料液比33:1时，多糖得率达到极大值。该条件下多糖得率预测值为12.59%，验证值为12.63%。

Abstract:

The extraction of polysaccharides from the residue of pueraria after extraction of Flavonoids was studied in this paper. According to the single-factor experiments, three levels of the factors (extraction temperature, time and the ratio of water to pueraria residue) were selected for the central composite design to establish a quadric regression equation for describing the yield of polysaccharides. By response surface analysis, the optimum extraction conditions were obtained as follows: extraction temperature of 98℃, time of 2.4 h and water-pueraria residue ratio of 33:1, under which the highest yield of polysaccharides reached with estimated value and verified value being of 12.59% and 12.63%, respectively.