研究从毛杨梅树皮中提取杨梅醇的工艺条件及用HPLC法测定其含量的色谱条件。实验数据显示，以甲醇作为溶剂，提取时间45 min，提取效率最高，可达4.78%。采用Dima ODS柱（4.6 mm250 mm，5 µm），流动相为V乙睛:V水=80:20，体系流速为1mL/min，紫外检测器254 nm的检测条件下，以实验室制备的杨梅醇为标准品，在2.552~12.76 μg，范围内，相关系数为1.00，平均回收率为100.1%。研究结果表明，该方法简便可行、精密度高、稳定性强、重复性好，可作为毛杨梅资源的进一步合理开发的依据及其质量评价的方法。

The extraction conditions of myricanol in Myrica esculenta bark were studied and its content was determined by HPLC. The highest extraction rate of myricanol reached 4.78% by methanol extraction for 45 min. Gradient elution of the extract was performed on a Dima ODS (4.6 mm×250 mm, 5 µm) column using UV detector at 254 nm. A mixture of acetonitrile and water (80:20 by volume) was used as mobile phase at a flow rate of 1 ml/min. Within a linear range of 2.552~12.76 μg, the coefficient and RSD were of 1 and 100.1%, respectively. This method was simple, reliable and reproducible with highly sensitive. It can be used for the quality evaluation of Myrica esculenta resources for their further development.