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[摘要]
建立了应用PCR技术鉴别杏仁产品中的杏仁成分的方法。杏仁产品经前处理后,用酚仿抽提DNA,提取时间为2 h左右,提取所得的DNA浓度和A260/A280均达到了PCR的要求。该方法快速简便,不仅可以检测杏仁产品的DNA,且可检测到杏仁产品中0.1%的其它植物成分。
[Key word]
[Abstract]
In this paper, a PCR-based method was developed for detection of amygdaline components in food. The pre-treated samples were extracted by phenol-chloroform for 2 h, and then the achieved DNA extracts from amygdaline foods were amplified by PCR. Results showed that this method was suitable for the detection of amygdaline components.
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