优良醋酸菌种AC2005的鉴定

Identification of high Acidic acid-producing Strain of Acetobactor AC2005

摘要:

将醋酸杆菌AC2005 16S rDNA片段转入载体pMD18-T，并转化大肠埃希氏杆菌（Escherichia coli）。经测序和序列分析显示，醋酸杆菌AC2005 16S rDNA片段与巴斯德醋杆菌16S rDNA片段同源性均达95%。根据醋酸杆菌AC2005 16S rDNA的生理生化特征，培养特征，形态特征结果将醋酸杆菌AC2005归属为巴斯德醋酸杆菌罗旺亚种（Acetobactor. pasteurianus subsp.lovaniensis）。

Abstract:

The 16Sr DNA sequence of Acetobacter AC2005 was transferred into plasmid pMD18-T after amplified by PCR. Then the recombined plasmid was transformed in Escherichia coli. The analysis of 16S rDNA sequence indicated that the sequence was similar to the 16 Sr DNA sequence of Acetobactor pasteurianus with the homology up to 95%. According to the differences in biochemical characteristics, incubation characteristics, conservative sequence contrast, the strain was further identified as A. pasteurianus subsp.lovaniensis.