甲基苯丙胺与蛋白偶联物的合成

Synthesis of Methamphetamine-Bovine Serum Albumin Conjugates

摘要:

研究合成甲基苯丙胺抗原。在甲基苯丙胺分子上引入偶联官能团，以牛血清白蛋白（BSA）为载体蛋白，用戊二醛法偶联合成了甲基苯丙胺抗原，并用红外光谱、紫外扫描、蛋白电泳以及胶体金免疫层析等方法进行检测。结果表明，每分子BSA连接甲基苯丙胺分子数为25；胶体金免疫层析检测结果显示合成抗原与抗体有特异性反应，具有活性。可在免疫检测中用作抗原的甲基苯丙胺与蛋白偶联物。

Abstract:

To synthesize the methamphetamine antigen for immunoassay. Functional group was introduced into methamphetamine and then methamphetamine antigen was synthesized by anhydride method using BSA as carrier protein. The reaction was monitored by FTIR, UV scanning, protein electrophoresis and GICA methods. Results: 25 molecules of methamphetamine were conjugated to one molecule of BSA. And the formed conjugate showed confirmed antigenic Activity. Methamphetamine antigen was successfully synthesized and could be used in immunoassay.