油茶饼粕发酵条件的研究

Fermentation Conditions for Single Cell Protein Feed Production from Oil-tea-cake

摘要:

以油茶饼粕和血粉为主要原料，以黑曲霉、米曲霉、绿色木霉和产朊假丝酵母组成的混合菌种为发酵剂，研究了油茶饼粕进行菌体蛋白饲料生物转化时发酵条件和培养基原料配比对发酵产物菌体干重的影响。通过正交实验，确定最佳培养基配比为：m茶粕:m血粉＝17:3，m料:V水＝1:1.2，初始pH为5.0~5.5；最佳发酵条件为：250 mL三角瓶，装料量15 g，总接种量10%，其中霉菌与酵母菌的接种比为1:1，培养时间为4 d。在最佳培养基和发酵条件下，得到的菌体干重为一最大值21.25 g/L。

Abstract:

The producing conditions of single cell protein feed from oil-tea-cake were studied by solid state fermentation. The optimal culture conditions were as follows: ratio of oil-tea-cake to swine blood power of 17:3, ratio of fermenting medium to water of 1:1.2, (NH4)2SO4 content of 2.0% and initial pH value of 5.0~5.5. The fermentation conditions were also optimized as follows: in 250 mL of a triangular flask, the loading volume of the fermentation medium of 15 g, inoculation size of 10%, the ratio of mildew to yeast of 1:1 and fermentation time of 4day. Under those conditions, the maximum dry cell weight was 21.25 g/L.