栀子蓝色素制备及纯化工艺的研究

Isolation and Purification of Gardenia Blue Pigment with Ultra Filtration

摘要:

本文采用纤维素酶水解京尼平甙生成京尼平，京尼平再与氨基酸合成栀子蓝色素，利用超滤技术对其进行分离、纯化。通过一系列的单因素实验和正交实验，探讨了制备及纯化栀子蓝色素的工艺条件。结果表明，最佳工艺条件为以谷氨酸钠作为氨基酸来源，液固比（V/m）=8:1，酶解时间6 h，纤维素酶与京尼平甙的质量比1:8，氨基酸与京尼平甙的质量比1:2，反应时间96 h。最佳超滤纯化工艺为超滤膜截至分子量为5000 Da，超滤压力0.5~0.8 MPa，pH 7，超滤温度为室温。经HPLC测定，经本工艺制备的栀子蓝色素色价E590 nm1 cm（1%）≥192，纯度≥95%。

Abstract:

In this study, gardenia blue pigment was synthesized using amino acids and genipin which was produced via cellulose-catalyzed hydrolysis of geniposide. And the isolation and purification of the achieved pigment by ultra filtration were optimized by single factor test and orthogonal test. The results indicated that the most suitable amino acid, ratio of liquid to solid, the enzymatic hydrolysis time, molar ratio of cellulase to geniposide, molar ratio of amino acids to geniposide and reaction time were Glu, 8:1(v/w), 6 h, 1:8(g:g), 1:2(g:g), and 96 h, respectively. The best purification conditions were as follows: the retention molecular weight of 5000 Da, operating pressure of 0.5~0.8 MPa, room temperature and pH value of 7. Under those conditions, the gardenia blue pigment was achieved with high E590 nm1 cm (1%) value (≥192) and purity (≥95%).