从大豆磷脂中分离高纯脑磷脂的研究

Extraction and Purification of Cephalin from Soybean Phospholipid

摘要:

研究了用有机溶剂法从大豆磷脂中提取制备高纯度脑磷脂的工艺，大豆磷脂先用丙酮萃洗溶解去除其中的油脂和游离脂肪酸，然后用无水乙醇溶解除去卵磷脂，最后用石油醚萃取沉淀物得高纯度的脑磷脂。对石油醚萃取高纯度脑磷脂的工艺进行了探讨，得到最佳工艺参数为：料液比为1:4（w/V）；萃取次数为3次；萃取温度为30 ℃。在此工艺条件下脑磷脂的得率为91.9%，纯度为93.5%。

Abstract:

The extraction and purification of cephalin from the soybean phosphatide were studied in this paper. After washed by acetone to remove greases and free fatty-acids, the soybean phosphatide was treated by ethanol to remove lecithin and then extracted by petroleum ether. For extraction of cephalin with high purity，the best technological parameters were determined as follows: the ratio of solid to liquid ratio of 1:4 (W/V), extract times of 3, and extract temperature of 30 ℃. Under those conditions, the yield of cephalin was 91.9% with the purity of 93.5%.