海产品中溶藻弧菌的筛选及其致病因子的研究

Screening of Vibrio Alginolyticus in Sea Foods and their Pathogenic Factors

摘要:

溶藻弧菌能引起人类食物中毒。溶血毒素、耐热性肠毒素（ST）和不耐热性肠毒素（LT）是致病性微生物中常见的致病因子。本文对从扇贝、牡蛎、贻贝、沙虾、虾蛄等海产品中筛选的溶藻弧菌的种属和致病因子进行了研究，生化鉴定显示分离到的两株溶藻弧菌来源不同，基因测定显示它们同属于溶藻弧菌（V. alginolyticu s）；血琼脂平板法、兔小肠结扎法和乳鼠灌胃法显示来源不同的溶藻弧菌其产毒种类也不同，两株溶藻弧菌都能产生不耐热性肠毒素（LT）和耐热性肠毒素（ST），但只有1株能产生溶血毒素。

Abstract:

Hemolysin, heat-stable eterotoxin (ST) and liable- heat enterotoxin (LT) were common pathogenic factors in pathogeinc microbes, such as Vibrio Alginolyticus. In this study, two strains of Vibrio Alginolyticu were screened from several kinds of sea foods and identified by different methods. Both of the two strains could produce LT and ST, one of which showed hemolysin activity. It was inferred that Vibrio alginolyticus from different sources could produce different toxins.