高效液相色谱-荧光法测定茶叶中苯并（α）芘残留量的研究

Determination of Benzo (α) Pyrene Residue in Tea by HPLC with Fluorescence Detection

摘要:

建立了茶叶中苯并（α）芘残留量的高效液相色谱-荧光测定方法。样品用固相萃取小柱净化，采用高效液相色谱分离，荧光检测器检测。苯并（α）芘在0.2 g/L~50 g/L范围内呈线性，线性方程为y=3.85×104x+7.47×103，相关系数为0.9991。分别对绿茶和红茶进行添加回收试验，回收率在76.9%~86.9%之间，相对标准偏差在2.0%~4.0%之间，方法检出限为0.4 μg/kg。结果表明该法简便、灵敏、安全性好、线性范围宽、精确度高，适用于茶叶中苯并（α）芘的残留分析。

Abstract:

A method for the determining of Benzo (α) pyrene residue in tea by HPLC with fluorescence detection (HPLC-RF) was developed. Samples were purified by SPE, and analyzed by HPLC-RF. Within the linear range of 0.2~50 g/L, the linear equation was Y=3.85×104X+7.47×103 (R=0.9991). The recovery tests of green and red teas ranged from 76.9% to 86.9%. The variation coefficients and detection limit were 2.0%~4.0% and 0.2 μg/kg (S/N=3), respectively. This method was suitable for the qualitative and quantitative analysis of Benzo (α) pyrene residues in tea, due to its simpleness, sensitivity, safety and high precision.