双酶法生产葡萄糖工艺优化研究

Optimization of Glucose Production by Two Enzymes

摘要:

通过对双酶法制糖工艺进行优化试验。结果得出最佳工艺为：喷射温度108~110 ℃、液化pH 5.4~5.8、喷射器后高温维持时间5~7 min、淀粉酶总量为0.6 kg/t、分40%和60%两次添加、粉浆浓度＞16 °Be时尽可能选用进口耐高温α－淀粉酶和新型水热器、糖化时适当添加脱支酶等。

Abstract:

The process of glucose production by two enzymes was studied. The processing conditions for glucose production were optimized and the best spurt temperature, liquefaction pH value, treat time at high temperature after rejection and total amylase amount were 108~110 ℃, 5.4-5.8, 5~7 min and 0.6 kg/t, respectively. It was also found that α-amylase with high thermal stability and new hydroheater should be adopted when the starch concentration was＞ 16 °Be. Besides, debranchins enzyme should be added during saccharification.