烟草绿原酸分离纯化及其抑菌性研究

Study on Extraction, Purification and Antimicrobial Activity of Tobacco Chlorogenic Acid

摘要:

研究了烟草绿原酸提取纯化工艺条件及其抑菌效果。采用超声波提取、大孔吸附树脂纯化烟草绿原酸，抑菌圈直径法测量绿原酸抑菌水平。结果表明：最佳提取条件为乙醇浓度60.5%，超声波处理时间30.4 min，温度51.4 ℃，绿原酸得率1.6%；NKA-9型大孔吸附树脂对绿原酸有较好的吸附及解吸效果，均达70%以上；烟草绿原酸对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌均具有一定的抑菌效果。

Abstract:

The extraction, purification and antimicrobial activity of chlorogenic acid in tobacco leaves were researched. The optimal ultrasonic-assisted extraction conditions were as follows: 60.5% ethanol, 51.4 ℃ and 30.4 min. Under these conditions, the yield of crude tobacco chlorogenic acid was 1.6%. The crude chlorogenic acid was purified by NKA-9 resin. It was also found that the tobacco chlorogenic acid had inhibition activities on Escherichia coli, Staphylococcus aureus and Bacillus subtilis.