紫外线与氯化锂复合诱变选育L-组氨酸产生菌

Mutation Screening of L-histidine Producing Strain by UV and LiCl

摘要:

以一株谷氨酸棒杆菌（Corynebacterium glutamicum）S6为出发菌株，利用氯化锂、紫外线进行诱变，通过实验证明氯化锂诱变剂量在1.2%时致死率达到82.4%，紫外线在照射30 s时致死率达到81.8%。利用氯化锂诱变谷氨酸棒杆菌S6，所得菌株D3的L-组氨酸产量为243 mg/L，比出发菌株提高10.5%；以紫外线做诱变S6，所得高产菌株U1产量256 mg/L，比出发菌株提高8.5%；以紫外线、氯化锂复合诱变S6，所得菌株N1产量为251 mg/L，比出发菌株提高13.6%，结果显示，经紫外线与氯化锂复合诱变后的菌株N1产L-组氨酸的产量最高，比氯化锂诱变后的菌株产L-组氨酸量提高3.1%，比紫外线诱变后的菌株产L-组氨酸量提高5.1%。

Abstract:

A L-histidine producing strain was mutated and screened from Corynebacterium glutamicum S6 by LiCl and UV. Results showed that the UV-induced lethality was 81.8% with the irradiating time of 30s, while the LiCl-induced lethality was 82.4% with the LiCl concentration of 1.2%. Using three mutants, D3 by LiCl, U1 by UV, and N1 multiple mutated by UV and LiCl, the L-histidine yields reached 256 mg/L, 243 mg/L and 251 mg/L, respectively, which were 8.5%, 10.5% , and 13.6% respectively higher than that by the original strain S6.