反相高效液相色谱同步对酸橙汁中柚皮柑和橙皮柑的定量分析研究

Quantitative Analysis of Narigin and Hesperidin contents in Citrus Aurantium Juice by Reverse-phase High-performance Liquid Chromatography

摘要:

研究了RP-HPLC同步测定酸橙汁中的柚皮柑和橙皮柑法的色谱条件，并对甲醇溶解+超声处理与水溶解+静置处理进行比较，并对结果进行准确度和精密度试验。其色谱条件为V甲醇: V水=40:60，固定相ODS-C18，柱子4.6 mm´150 mm，流速1 mL/min，检测波长285 nm；甲醇溶解+超声处理测得的酸橙汁中柚皮苷、橙皮苷含量高于水溶解+静置处理，含量分别提高了32%和4.4%；试验操作简便，结果准确可靠。

Abstract:

The chromatographic conditions for quantitative analysis of narigin and hesperindin contents in citrus aurantium juice were investigated and the optimal reverse-phase chromatogram conditions were found as follows: methanol/water being of 40/60, column being of ODS-C18 (4.6 mm´150 mm), flow rate of 1mL/min and the detection wavelength being of 285 nm. It was also found that the contents of narigin and hesperidin in citrus aurantium juice via methanol dissolve and supersonic treatment were 32% and 4.4% higher than those via water dissolve and standing storage, respectively. The method was simple and accurate.