胶体金免疫渗滤法检测淡水鱼及牛乳中氯霉素残留

Detection of Chloramphenicol Residue in Freshwater Fish and Milk by Dot Immunogold Filtration Assay

摘要:

制备了平均粒径20 nm的胶体金，用以标记抗氯霉素多克隆抗体；将氯霉素半抗原与卵清蛋白的偶联物包被在硝酸纤维素膜上作为检测带，羊抗兔二抗作为质控带，依据免疫竞争法原理，建立通过免疫胶体金渗滤式试纸条检测六种淡水鱼及牛乳中氯霉素残留的方法。样品前处理方法简便，方法灵敏度为1 μg/L，只需10~15 min即可目测判断结果，可以作为淡水鱼及牛乳中氯霉素残留现场大量筛选的有效手段。

Abstract:

An immunogold assay for detection of chloramphenicol (CAP) residue in freshwater fish and milk was established using the colloidal gold with the average diameter of 20 nm for labeling the anti- chloramphenicol (CAP) polyclonal antibody. CAP was conjugated to (OVA) and then they were dispersed on a nitrocellulose (NC) membrane forming the test zone. Goat anti-rabbit IgG was chose as control zone. The method was simple and fast (10~15 minutes) with the sensitivity of 1 μg/L, which could be widely used to detect the CAP residues in freshwater fish and milk.