微波辅助提取甘蔗糖厂滤泥中叶绿素的初探

Extraction of Chlorophyll from Filter Mud with Microwave Pretreatment

摘要:

初步探讨了应用微波从甘蔗糖厂滤泥中辅助提取叶绿素的工艺，结果表明，用5 mL 60%乙醇溶解5 g干滤泥，650 W的微波照射50 s后加入50 mL 95%乙醇溶液于60 ℃下萃取30 min，提取液中叶绿素含量达到45 mg/mL。与传统提取方法相比较，提取率比传统方法的23.86 mg∕mL明显提高，提取时间比传统方法的4~12 h大大缩短。

Abstract:

Extraction process of chlorophyll from the microwave radiated filter mud was investigated. The best procedure was as follows: after adding 5 mL of 60% alcohol to dissolve 5 g of dried filter mud, the mixture was pretreated by microwave, and then extracted for 30 min by 50 mL of 95% alcohol at 60 ℃. Under the above-mention conditions, the chlorophyll content reached 45 mg/mL, which was higher than that by traditional method (23.86 mg∕mL). And the extraction time was shorter than that of the traditional method (4~12 h).