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[摘要]
初步探讨了应用微波从甘蔗糖厂滤泥中辅助提取叶绿素的工艺,结果表明,用5 mL 60%乙醇溶解5 g干滤泥,650 W的微波照射50 s后加入50 mL 95%乙醇溶液于60 ℃下萃取30 min,提取液中叶绿素含量达到45 mg/mL。与传统提取方法相比较,提取率比传统方法的23.86 mg∕mL明显提高,提取时间比传统方法的4~12 h大大缩短。
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[Abstract]
Extraction process of chlorophyll from the microwave radiated filter mud was investigated. The best procedure was as follows: after adding 5 mL of 60% alcohol to dissolve 5 g of dried filter mud, the mixture was pretreated by microwave, and then extracted for 30 min by 50 mL of 95% alcohol at 60 ℃. Under the above-mention conditions, the chlorophyll content reached 45 mg/mL, which was higher than that by traditional method (23.86 mg∕mL). And the extraction time was shorter than that of the traditional method (4~12 h).
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