大孔吸附树脂法纯化杭白菊总黄酮

Purification of Total Flavonoids from HangZhou White Chrysanthemum using Macroporous Absorption Resin

摘要:

采用优选树脂纯化杭白菊总黄酮，以分光光度法测定杭白菊总黄酮含量。确定大孔吸附树脂纯化杭白菊总黄酮的最佳工艺条件；AB-8树脂纯化杭白菊总黄酮的工艺参数为：树脂最大静态吸附容量为 23.06 mg/g（黄酮/树脂），树脂吸附率为89.22%，提取液上样后吸附1 h，用4 BV的70%乙醇进行洗脱，洗脱率可达到96.64%；结果表明，AB-8树脂对杭白菊总黄酮具有良好的吸附和纯化性能。

Abstract:

The total flavonoids of Hangzhou white chrysanthemum was purified by macroporous absorption resin and its content was determined by UV spectrophotometry. The optimal purification conditions by AB-8 macroporous absorption resin were as follows: maximum static adsorption capacity of 23.06 mg/g, resin adsorption rate of 89.22%, absorption time of 1 h. Besides, 4BV of 70% alcohol was used as eluent with an eluent rate of 96.64%. AB-8 resin showed good absorption and purification capability of the total flavonoids from HangZhou white chrysanthemum.