古尼虫草多糖提取分离及初步分析

Extraction, Isolation and Analysis of the Polysaccharides from Cordyceps Gunnii (Berk.) BerK.

摘要:

本文用30%乙醇溶液作提取剂，从古尼虫草菌丝体中提取多糖，提取液经去脂、去蛋白、醇析后得两种粗多糖CPS-50，CPS-70。利用DEAE-Sephadex A-25柱色谱对两种粗多糖进行分离纯化，得纯多糖CPS-1，CPS-2。苯酚-硫酸法测定糖含量，紫外光谱分析证明其纯度，红外光谱显示其具有多糖类的特征吸收峰。多糖降解后经薄层分析表明，降解的虫草多糖由葡萄糖或（和）甘露糖组成。

Abstract:

Two crude polysaccharides, CPS-50 and CPS-70, were extracted from the mycelium of Cordyceps gunnii (Berk.) Berk. using 30% ethanol. After remove of lipids (by ethyl acetate) and proteins (using sevage method), and precipitation (with 50% and 70% alcohol), the crude polysaccharides were further purified by DEAE-Sephadex A-25 column. The contents and purities of the obtained polysaccharides, named as CPS-1 and CPS-2, were determined using phenol-sulfuric acid method and UV spectrophotometric method, respectively. Their structures were analyzed by infrared spectrum (IR). In addition, thin layer chromatography analysis showed that the hydrolysis of the pure polysaccharides yielded two kinds of monosaccharides, glucose and/or mannose.