古尼虫草菌丝体维生素E的初步分析

Primary Study on the Vitamin E of Cordyceps gunnii (Berk.) Berk.

摘要:

古尼虫草与传统名贵中药冬虫夏草类似，具有多种活性物质及广泛的药理作用，是一种重要的虫草属真菌。分别用30%、50%、80%乙醇溶液和乙酸乙酯作为提取液，从古尼虫草菌丝体中提取维生素E，并用乙酸乙酯萃取的方法进行初步分离，硅胶薄层层析和紫外分光光度法分析粗产物中的维生素E。结果表明，提取物质的总量与提取液的乙醇含量有关，提取液的有机溶剂比例越高，提取到维生素E量越高，但提取的维生素E的纯度越低。初步确定乙酸乙酯为提取虫草维生素E的最适溶剂。

Abstract:

Cordyceps gunnii (Berk.) Berk. is one of the important species of Cordyceps spp, which has similar constituents and pharmacological activities to the traditional Chinese medicine Cordyceps sinensis. In the paper, the extraction and separation of vitamin E from Cordyceps gunnii (Berk.) Berk. were studied using 30%, 50%, 80% ethanol solution and ethyl acetate. The content of the crude vitamin E was analyzed with thin layer chromatography (TLC) and UV spectrophotometer methods. The results showed that the higher the organic solvent content in the extracting solution, the higher the content of vitamin E and the lower the purity of the vitamin E. Besides, ethyl acetate was shown to be the best organic solvent for the extraction of vitamin E from Cordyceps gunnii.