低钠盐饮食对血压调控相关基因局部网络影响的初步研究

Preliminary Investigation of the Effect of Low-Sodium Intake on Hypertention-related Gene Network

摘要:

目的：调查食用低钠盐饮食对血压调控相关基因的表达的影响。方法：确定正常组和疾病组两组测试人群各3人，食用低钠盐两个月。抽提测试人群食用低钠盐前后外周血中的总RNA，经反转录应用22 K Human Genome Array芯片分析基因表达差异。结果：在利用基因芯片监测的150个高血压候选基因中，正常组和疾病组人群的部分基因表达都有差异（比值大于2为表达升高，小于0.5为表达降低）。在疾病组测试人群中ADA、NPRB基因3人共同表达降低，正常组和疾病组SLC8A1基因都表达升高。结论：根据本实验观测，通过减少钠盐的摄入, 离子通道和转运通路等方面对应的局部基因网络状态发生改变，为低钠盐成为降压非药物治疗的有力补充在基因表达层面上提供了证据。

Abstract:

Objective: to investigate the effect of low-sodium intake on the expression of hypertention related genes. Method: Six people were apportioned into a control and a patient group (n=3 each) to take the low-sodium intake for two months. Their total RNA samples were extracted, purified and treated by reverse-transcription. Then the gene expression profiles were analyzed by 22 K Human Genome Array experiments. Result: Among the 150 hypertension candidate genes, differences in some gene expression were found in both of the examined groups (the ratio of >2 and<0.5 were regarded as up-regulated and down-regulated expression, respectively). The expression levels of ADA and NPRB genes were decreased in the patient group, while that of the SLC8A1 gene increased in both the groups. Conclusion: Our preliminary investigations provided evidence that consumption of low-sodium salt could adjust local network status of some hypertension candidate genes functionally linked with iron channels/transporters.