薄层色谱-分光光度法测定葵花籽粕中的绿原酸

Determination of Chlorogenic Acid in Sunflower Kernel by Thin Layer Chromatography-spectrophotometry

摘要:

建立了测定葵花籽粕中绿原酸的薄层色谱-分光光度法的新方法。用薄层色谱分离杂质，优化了分析条件，讨论了影响测定的因素。方法的线性回归方程为A=0.0451c+0.897，在0.20~1 μg/mL之间绿原酸的浓度与吸光度呈线性关系，相关系数r=0.9995。

Abstract:

A new method for determination of chlorogenic acid in sunflower kernel by thin layer chromatography-spectrophotometry was established. The chlorogenic acid in the sample was separated by thin layer chromatography. Analytical conditions of the chlorogenic acid were optimized and the effects of some key factors on the determination were discussed. Within the range of chlorogenic acid concentration of 0.20~1 μg/mL, the linear regression equation was A=0.0451c+0.897, with the linear correlation coefficient of 0.9995.