两种蛋白酶水解油松花粉蛋白的比较

Comparison of Hydrolysis of Chinese pine pollen Protein Catalyzed by two Proteases

摘要:

本文研究了碱性蛋白酶和木瓜蛋白酶水解油松花粉蛋白的工艺条件，对两种蛋白酶水解得到的肽进行了氮溶指数，清除•OH自由基和抗氧化能力方面进行了比较分析。其中，碱性蛋白酶水解得到的肽，其氮溶指数较高；木瓜蛋白酶水解得到肽，其清除•OH自由基和抗氧化能力较强。

Abstract:

The hydrolysis of the Chinese pine pollen protein by alkaline protease and papain was studied and the nitrogen solubility index, hydroxygen free radical-cleaning property and the antioxidant activity of the peptides achieved with two methods were compared. The peptide achieved by alkaline protease-catalyzed hydrolysis had higher nitrogen solubility index and lower hydroxygen free radical-cleaning property and antioxidant activity than that achieved by papain-catalyzed hydrolysis of Chinese pine pollen protein.