抗凝血纤维素硫酸酯制备过程的优化

Process Optimization for the Preparation of Anticoagulant Cellulose Sulfate

摘要:

对抗凝血纤维素硫酸酯制备过程进行五因素二次旋转正交实验和统计优化分析。结果表明，纤维素硫酸酯制备条件为酯化剂制备温度20 ℃、酯化剂用量6.8 mol/molAGU、反应温度12 ℃、反应时间3.5 h、pH 8.0，该条件下制备的纤维素硫酸酯对全血复钙时间（RT）、活化部分凝血活酶时间（APTT）和凝血酶时间（TT）的延长作用最强，即纤维素硫酸酯抗凝血活性最好。

Abstract:

The preparation of anticoagulant cellulose sulfate was investigated by five factors quadri-rotation orthogonal experiments and statistical optimization analysis. The results showed that the optimum preparation temperature of esterifying agent, esterifying agent dosage, reaction temperature, reaction time and pH value were 20.0 ℃, 6.8 mol/mol AGU, 12 ℃, 3.5 h and 8.0, respectively, under which, the cellulose sulfate had the highest effect on the prolongation of the RT, APTT and TT, meaning that the best anticoagulant activity of cellulose sulfate was achieved.