Alcalase水解大豆蛋白制备大豆蛋白寡肽的研究

Preparation of Soybean Oligopeptides by Alcalase-catalyzed Hydrolysis of Soybean Protein

摘要:

研究了Alcalase水解大豆蛋白制备大豆蛋白寡肽时影响水解效果的各种因素，在此基础上通过正交试验确定了Alcalase水解大豆蛋白的水解条件：温度70 ℃，底物浓度60 g/L，Alcalase酶与底物比为20 μL/g，pH 7.5，水解时间为4 h。在此水解条件下，水解液水解度达到了24.1%。

Abstract:

The effects of several factors on the alcalase-catalyzed hydrolysis of soybean protein, such as the enzyme dosage, temperature, pH value and reaction time, were studied. The orthogonal test showed that the optimized hydrolysis conditions were as follow: pH value of 7.5, reaction temperature of 70 ℃, alcalase dosage of 20 μL/g protein, substrate concentration of 60 g/L, and total reaction time of 4 h. Under these conditions the hydrolysis degree of soybean protein reached 24.1%.