磁性亲合分离法纯化胰蛋白酶

Magnetic Affinity Support for Trypsin Separation and Purification

摘要:

采用壳聚糖包埋纳米钡铁氧体，经环氧氯丙烷交联制得磁性亲和载体，应用激光粒度分析仪和振动样品磁性测定仪对磁性亲和载体进行表征，动态激光散射分析结果表明，磁性粒子的平均直径为401纳米。磁性测量表明，超细磁粉具有较高的磁性。然后利用碳二酰亚胺活化法，将抑肽酶与此微粒共价结合得到磁性亲和吸附剂，应用于胰蛋白酶的亲和纯化。论文详细讨论胰蛋白酶亲和纯化条件，将磁性亲合分离法成功地应用于亲和纯化胰蛋白酶。

Abstract:

Ultrafine high magnetic affinity support was prepared by coating nanometer barium ferrite with chitosan, cross-linking with epichlorohydrin, and then activating with carbodiimide. The analysis result of the dynamic laser scatters indicated that the average diameter of the magnetic particles was 401 nm. The magnetic measurement showed that the ultrafine magnetic particles were high magnetic. At first the aprotinin was considered to be affinity ligand and covalently bound onto the magnetic particles via carbodiimide activation, and then these particles were used for the affinity purification of trypsin. The proposed method was successfully applied to the affinity purification of trypsin with the purified conditions of trypsin affinity under discussing.