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[摘要]
研究了四种植物油(豆油、棕榈油、花生油、芝麻油)对被孢霉菌发酵生产花生四烯酸(Arachidonic acid,AA)的影响。结果表明:(1)四种植物油显著促进了菌体油脂的积累;(2)被孢霉生长在以植物油为唯一碳源的培养基上时,菌体油脂中的AA含量比以葡萄糖为碳源时显著下降;生长在以植物油和葡萄糖为混合碳源的培养基上时,与以葡萄糖为碳源相比油脂中的AA含量仍然下降,但比生长在以植物油为唯一碳源时AA含量有所提高。说明这四种植物油对花生四烯酸的合成有抑制作用。(3)菌体油脂成分与培养基中植物油的油脂成分有一定的相似性。
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[Abstract]
The Effects of addition of four kinds of vegetable oils (soybean oil, palm oil, peanut oil and sesame oil) on arachidonic acid production by Mortierella sp. were studied. The results indicated that vegetable oils markedly enhanced the mycelial lipid accumulation. When Mortierella sp. was cultured in medium with vegetable oils as single carbon source, arachidonic acid content in lipids of the fungi greatly decreased compared with that using glucose as single carbon source. For that cultured in medium with vegetable oil and glucose as carbon sources, the arachidonic acid content was also lower than that using glucose as single carbon source, but higher than that using vegetable oils as carbon source. These pheromones indicated that vegetable oils inhibited the biosynthesis of arachidonic acid. In addition, the composition of the mycelia lipid was similar to the vegetable oil added in the medium.
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