珠母贝糖蛋白分离提取工艺条件优化

Extraction of Glycoprotein from Pinctada martensii

摘要:

采用单因素实验与正交实验，研究珠母贝全脏器中糖蛋白的分离提取工艺条件。结果表明：珠母贝全脏器中糖蛋白的最佳分离提取条件为：以1.0 mol/L NaCl溶液为浸提剂，料液比为1:10，浸提温度为65 ℃，浸提时间为60 min，提取液经透析浓缩、醇沉后得到珠母贝糖蛋白，得率为3.37%，总糖和总蛋白含量分别为7.49%和25.33%。

Abstract:

The extraction of glycoprotein from the whole viscera of the Pinctada martensii was studied. The results showed that the optimal separation conditions were NaCl concentration of 1.0mol/L, extraction temperature of 65 ℃ and extraction time of 60 min. The crude extracts were dialyzed with water, concentrated and then precipitated with alcohol, giving pure coarse glycoprotein with the yield of 3.37%. The content of total sugar and total protein in the coarse glycoprotein were 7.49% and 25.33%, respectively.