[关键词]
[摘要]
本研究通过参考文献,直接使用常见油料植物种子制备纯油甲酯进行分析,探讨了快速制备甲酯的试剂选择及用量,强极性和弱极性的两种不同类型毛细管柱的最佳色谱分析条件,并将测定的主要脂肪酸含量与国家标准进行比较,结果较为一致,而且平行测定的相对标准偏差表明方法有较好的精密度和重复性。本论文所测ECL值与文献标准值对照,差别很小,说明本实验操作系统是稳定和可靠的,本研究试图得到一种能够检测大批量样品的简单、快速、准确的植物油脂肪酸组成分析方法。
[Key word]
[Abstract]
Pure methyl esters were prepared using common oil plant seeds and the fatty acid composition of the achieved oil was measured by capillary column chromatography. The kinds and dosage of reagents for fast preparation of the methyl esters were investigated and the chromatographic conditions using two kinds of capillary columns were optimized. Under the determined conditions, the fatty acid contents in the oil were in accord with the national standards. Compared the standards values with ECL values, the method was steady and credible, because the difference of comparison was little. This simple, fast, accurate method was suitable for determining fatty acid composition of samples in large quantities.
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