正交试验法优选艾叶总黄酮提取工艺研究

Research on Ultrasonic Wave Extraction of Total Flavones from Folium Artemisiae Argyi

摘要:

本文为测定艾叶中总黄酮的含量采用，设计正交设计优选艾叶中总黄酮的提取条件，用芦丁作对照品，硝酸铝作显色剂，测定波长在510 nm处，比色法测定艾叶总黄酮的吸光度。实验结果获得优选提取条件是：用70 mL 60%的乙醇浸泡1.000 g左右的艾叶粉末24 h，在超声波功率为200 W的条件下，辅助萃取40 min。结论：艾叶中含有丰富的黄酮类化合物，具有很强的抗氧化活性，认为艾叶有很强的开发应用价值。

Abstract:

Object: To investigating the optimum extracting conditions of the total flavones in Folium artemisiae argyi. Methods: The extracting conditions of the total flavones in folium artemisiae argyi by ultrasonic waves were optimized by the single-factor and orthogonal experiments. Using rutin as control reagent and aluminium nitrate as colorimetric reagent, the absorbency of total flavones was determined with colorimetric wavelength at 510 nm. Results: The best dipping time in 60% alcohol, ulgtrasonic wave power and extraction time were 24 h, 200 W and 40 min, respectively. Conclusions: The flavonoid compounds were in abundance in Folium Artemisiae Argyi and had strong anti-oxidant activity. The results also indicated a promising prospect fro development of Folium Artemisiae Argyi.