灵芝液体培养菌株的选择及其发酵条件优化

Studies on Screening of the Strains for Production of Ganoderma Lucidum Polysaccharide by Submerged Fermentation and Optimizating the Culture Conditions

摘要:

在适于液体培养的灵芝菌株中，经选择试验确定赤芝Ｇ22为液体发酵生产多糖的高产菌株，并对赤芝Ｇ22菌株的液体发酵条件进行了选择优化。结果表明，实现多糖高产的发酵工艺条件为：发酵温度28 ℃、摇床转速180 r/min、培养基初始pH值6.0、发酵时间144 h。采用此优化的工艺条件进行液体发酵，使灵芝多糖产量由1.823 g/L提高到2.315 g/L，提高了26.98%。

Abstract:

The capacities of the Ganoderma lucidum strains for production of Ganoder Lucidum Polysaccharide (GLP) by submerged fermentation were examined and Ganoderma lucidum G22 was shown to be the best one. The fermentation conditions for GLP production by Ganoderma lucidum G22 were also studied and the optimal fermenting temperature, shaking rate, the initial pH value of the medium and fermenting time were 28 ℃, 180 r/min, 6.0 and 144 h, respectively, under which the yield of GLP was improved to 2.315 g/L.