莱克多巴胺抗体的制备与评价

Production and Evaluation of a Antibody Against Ractopamine

摘要:

本文以RAC-linker-BSA为免疫抗原获得了莱克多巴胺的多克隆抗体，用混合酸酐法合成包被抗原，建立了莱克多巴胺的间接竞争酶联免疫检测方法（ELISA），并对抗体性质进行了评价。结果表明，制备出的莱克多巴胺抗体具有较高的灵敏度，IC50为0.9 ng/mL，检出限（IC15）为0.1 ng/mL，低于同类文献的报道结果；除了与多巴酚丁胺的交叉反应为7.5%外，与克伦特罗、沙丁胺醇、特步他林、异丙肾上腺素和异奎胍均无交叉现象，说明抗体特异性较高；此外，抗体具有很好的稳定性，可在4 ℃冰箱中储存半年以上。为建立酶联免疫快速检测方法，实现对莱克多巴胺的有效监控奠定了良好的基础。

Abstract:

An indirect competitive enzyme-linked immunosorbent assay (ELISA) procedure was established to detect ractopamine employing a polyclonal antibody generated from RAC-linker-BSA and a coating antigen synthesized by method of mixed acid anhydride. The antibody against ractopamine showed high sensitivity with an IC50 of 0.9 ng/mL and the detection limit (IC15) was 0.1 ng/mL, which was lower than the data reported in literatures. The antibody with high specificity showed no cross-reactivity with clenbuterol, salbutamol, isoproterenol, terbutaline and isoxsuprine, while had the cross-reactivity of 7.5% with dobutamine. In addition, it was stable enough to be stored at 4 ℃ for half a year. This study laid a well foundation for the control of ractopamine by ELISA method.