Abstract:The effects of ultrasonic treatment on hydrolysis of olive catalyzed by lipase and the enzymatic properties of lipase were researched. The results showed that, for lipase-catalyzed hydrolysis of olive, the optimal ultrasonic power, frequency and total ultrasonic time were 200 W, 20 kHz and 8 min, respectively. Under these conditions, the lipase activities were enhanced and its optimal temperature and pH value were not changed.