何首乌叶绿体matK基因序列分析

Study on the Genetic Variation of Polygonum multiflorum Thunb species by sequences analysis of chloroplast matK gene

摘要:

目的 通过DNA序列变异，初步研究何首乌品种的亲缘关系及其与地理分布的相关性。方法 采用PCR直接测序技术对来源于何首乌15个居群的17个样品的叶绿体matK基因进行测序分析研究。 结果 何首乌的matK基因长度均为1271bp，根据排序比较,何首乌17个样品间存在12个变异位点,用UPGMA（不加权的算术平均对群法）法构建的系统分支树将其分成四支。结论 matK基因序列可变位点为何首乌品种的归并提供了分子依据，且品种的亲缘关系与地理分布呈良好的相关性。

Abstract:

To investigate the relationship between the variation of chloroplast matK gene sequence and the geographical distribution of Polygonum multiflorum Thunb. Method: PCR direct sequencing was applied to detect the matK sequences of 17 samples of Polygonum multiflorum Thunb collected from 15 populations. Results: The matK gene sequences were 1271 bp in length. Multiple sequence alignment showed that there were 12 variable sites in matK sequences and the Polygonum multiflorum Thunb smples were divided into four groups. Conclusion: These relatively variable sites in matK gene provided potential information for identifying Polygonum multiflorum species at the DNA level. The phylogenetic relationship of Polygonum multiflorum Thunb was well correlated with their geographical distribution.