葡萄籽原花青素的分离纯化及其自由基淬灭活性的研究（下）

Study on Isolation, Purification and the Free-Radical Scavenging Effect of Proanthocyanidins from Grape Seed (2)

摘要:

本实验使用薄层扫描法进一步确认了梯度提取的乙酸乙酯、乙醚组分中原花青素单体含量，并与香草醛一盐酸法的结果加以对比，又用分光光度法考查了pH值、温度、光照以及用抗坏血酸和亚硫酸氢钠处理对原花青素稳定性的影响，最后用β-环糊精制备了原花青素微胶囊。结果发现，乙酸乙酯、乙醚组分中原花青素单体百分含量分别为29.02%和59.98%，与香草醛一盐酸法测定结果（33.25%和60.64%）基本一致；较低的pH值(pH 3~5)、避光、室温（20 ℃）有利于原花青素的保存，添加0.6%的抗坏血酸或0.75%亚硫酸氢钠对原花青素有很强的保护作用；β-环糊精、提取物用量分别为10 g和0.189 g时，微胶囊产率和包合效率分别为80.4%和20.5%。

Abstract:

The content of proanthocyanidins monomer extracted by ethyl acetate or ether was confirmed by thin layer chromatography scan (TLCS), and the results were compared with those by vanillin-HCI method. The effects of pH value, temperature, light, treatment with Vc and sodium hydrogen sulfite on the stability of proanthocyanidin in grape seeds were also investigated. Then the microcapsule of proanthocyanidins was made using β-cyclodextrin as coating material. The results showed that the contents of monomer in ethyl acetate and ether extracts by TLCS were 29.02% and 59.98%, respectively, which were in accordance with those by vanillin-HCI method. Proanthocyanidins was stable without light at low pH value (3~5) and room temperature. 0.6% Vc or 0.75% sodium hydrogen sulfite showed very strong protective effect on proanthocyanidins. When 10 g β-cyclodextrin and 0.189 g proanthocyanidins were used, the output and embedding efficiency of Proanthocyanidins microcapsule could reach 80.4% and 20.5%.