微生物多糖PS202发酵工艺条件的研究

Study on Fermentation of Polysaccharides PS202

摘要:

本文研究了产胞外多糖的PS202菌株的发酵条件，通过单因素试验及正交试验，得到PS202菌株产胞外多糖的最佳工艺条件是：蔗糖5%，牛肉膏0.2%，生长因子1.2%。pH 7.2～7.5，转速200 r/min，培养温度30 ℃，培养时间96 h。胞外多糖产量高达3.14%。

Abstract:

The fermentation conditions of Exopolysaccharides (EPSs) PS202 were determined by single-factor test and orthogonal test. From the results, the optimal sucrose content, beef extract content, the growth factor, pH value, shaking rate, temperature and extraction time for the fermentation were 5%, 0.2%, 1.2%, 7.2~7.5, 30 ℃, 200 r/min and 96 h, respectively. Under those conditions, the yield of EPSs was 3.14%.