RAPD技术在酿酒酵母分类鉴定与遗传育种中的应用

Application of RAPD in Molecular Classification and Genetic Breeding of Saccharomyces cerevisiae

摘要:

随机扩增多态性DNA（RAPD）具有快速、简便和灵敏等优点，在酿酒酵母的分子鉴定和分类、菌种分离纯化和污染检验、探索种属间亲缘关系和进化过程以及基因定位和辅助育种等研究中具有广阔的应用前景。本文对RAPD在酿酒酵母研究中的应用进行了综述，并且着重探讨了在上述研究应用中的缺点及改进方法。

Abstract:

The random amplified polymorphic DNA（RAPD）is a rapid and sensitive method for analysis of characters of microbe. There is a promising prospect for the application of this technology to molecular identification and characterization, separation, purification, research on genetic relation among species or genus, volution of yeast, functional gene location, and the assistant breeding of Saccharomyces cerevisiae. In this paper, the application of RAPD to molecular classification and genetic breeding of Saccharomyces cerevisiae was examined and its disadvantages and the corresponding improvement methods were also discussed