双酶法酶解文庆鲤蛋白的研究

Hydrolysis of Wenqing Carp Protein by two Enzymes

摘要:

同时加入枯草杆菌中性蛋白酶和木瓜蛋白酶对文庆鲤进行蛋白水解得到文庆鲤酶解蛋白的酶解率为71.21%。最佳工艺条件：温度50 ℃，前者酶量为2%，后者酶量为2.5%，m(底物):m(水)=1:2，酶解时间为5.5 h。文庆鲤酶解蛋白为优质蛋白制品。

Abstract:

Protein of Wenqing Carp was hydrolyzed by subtilisin and papain simultaneously. The yield of the hydrolysis was 71.21% under the optimum temperature, ratio of substrate to water, subtilisin dosage, papain dosage and hydrolysis time being of 50 ℃, 1:2, 2%, 2.5% and 5.5 h, respectively.