冠突散囊菌胞外多糖提取工艺研究

Extraction Techniques of Extracellular Polysaccharide of Eurotium cristatum

摘要:

冠突散囊菌是茯砖茶生产过程中形成茯砖茶独特品质的优势菌。从茯砖茶中分离纯化出冠突散囊菌,进行液体深层发酵，并对其产生的胞外多糖提取条件进行了正交试验。试验结果表明，本次试验中最优的多糖提取条件为：冠突散囊菌液体发酵液浓缩至原体积的1/4，乙醇浓度95%，乙醇沉淀时间10 h。

Abstract:

The Eurotium cristatum is the dominant germ in the production of Fuzhuan brick tea, contributing to the special quality of the tea. In this research, the Eurotium cristatum was separated from the Fuzhuan brick tea for submerged fermentation. Then the extracellular polysaccharides were separated after a 5-day liquid deep fermentation of the Eurotium cristatum. The results showed that the optimal ratio of condensed volume of fermentative liquor to the original, ethanol concentration and depositon time were 1:4, 95% and 10 h, respectively.