人工蛹虫草固体培养残基中虫草素的提取分离研究

Extraction and Isolation of Cordycepin from the Deserted Solid Medium of Cordyceps militaris

摘要:

以人工蛹虫草固体培养残基为原料，采用索氏提取后，用离子交换层析进行分离研究。索氏提取结果表明水比乙醇更优于提取虫草素；离子交换层析分离提取固体培养残基中虫草素的正交实验表明洗脱流速、料水比为主要影响因子，最优条件为：料水比116，离子柱pH 3.5；洗脱流速为1滴/2秒。

Abstract:

Extraction and isolation of cordycepin from the deserted solid medium of cordyceps militaris by soxhlet Extraction and ion exchange chromatography was studied. The soxhlet extraction showed that water was better than ethanol for the extraction of the cordycepin. The orthogonal test of ion exchange chromatography showed the main influential factors were flow rate of elution and ratio of material to water. The optimal ratio of material to water, the flow speed and the pH value were also confirmed to be 1:16, 3.5 and 1drop/2second, respectively.