[关键词]
[摘要]
以二氢查耳酮的含量为指标,研究了多穗柯(Lithocarpus polystachyus Rehd)叶中二氢查耳酮的纯化方法。结果表明二氢查耳酮纯化的适宜条件为:以聚合氯化铝为絮凝剂,在pH 7.5,温度为55 ℃时絮凝40 min;以ADS-7树脂为吸附剂,以100 mg/mL为上样液浓度,80%为乙醇为洗脱液浓度。用高效液相色谱法检测,二氢查耳酮的含量达99.02%。
[Key word]
[Abstract]
Using Dihydrocharcone content as the key parameter, the purification of Dihydrocharcone was optimized. The results indicated that the best flocculate reagent and sorbent reagent were Polyaluminum Chloride and ADS-7macroporous resin, respectively. HPLC analysis showed that the purity of the dihydrocharcone was 99.02%.
[中图分类号]
[基金项目]
广州市科技局项目(2006J1-C0251);广东省科技计划支持项目(2005A20303002 )