多穗柯中二氢查耳酮纯化的研究

Study on Purification Process of Dihydrocharcone from Lithocarpus Polysachyus Pehd

摘要:

以二氢查耳酮的含量为指标，研究了多穗柯(Lithocarpus polystachyus Rehd)叶中二氢查耳酮的纯化方法。结果表明二氢查耳酮纯化的适宜条件为：以聚合氯化铝为絮凝剂，在pH 7.5，温度为55 ℃时絮凝40 min；以ADS-7树脂为吸附剂，以100 mg/mL为上样液浓度，80%为乙醇为洗脱液浓度。用高效液相色谱法检测，二氢查耳酮的含量达99.02%。

Abstract:

Using Dihydrocharcone content as the key parameter, the purification of Dihydrocharcone was optimized. The results indicated that the best flocculate reagent and sorbent reagent were Polyaluminum Chloride and ADS-7macroporous resin, respectively. HPLC analysis showed that the purity of the dihydrocharcone was 99.02%.