黄皮新肉桂核酰胺B对C57BL/6小鼠的降血脂作用

Hypolipidemic Effect of Lansiumamide B Obtained from Wampee Seeds in C57BL/6 Mice

摘要:

从黄皮核中提取分离，得到单体黄皮新肉桂酰胺B（Lansiumamide B，LB），并进行结构鉴定，通过细胞和小鼠实验验证，LB具有较强的体内外降脂效果。结果表明：LB能高效抑制α-淀粉酶、α-葡萄糖苷酶和胰脂肪酶的活性，IC50分别为18.43、15.27和0.16 mg/mL；作用于油酸（OA）诱导的高脂HepG2细胞，浓度为5 μg/mL时，甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、谷丙转氨酶（ALT）、谷草转氨酶（AST）、丙二醛（MDA）分别降低了30.77%、30.00%、58.33%、39.97%、38.84%和43.90%，高密度脂蛋白胆固醇（HDL-C）、过氧化氢酶（CAT）以及低密度脂蛋白受体（LDLR）分别提高了83.33%、253.37%、60.97%；LB饲喂于高脂小鼠6周后，体重都有明显的降低，与高脂组有明显差异（P<0.05）；血清中的血脂四项，给药组剂量为60 mg/kg时，TC、TG和LDL-C分别降低了29.18%、23.69%、70.27%，HDL-C升高了50.51%，说明高脂小鼠的体重和血脂四项指标都得到了很好的控制。该研究初步表明LB具有较好的细胞和活体降血脂作用，是一种潜在的膳食降脂成分。

Abstract:

The monomer lansiumamide B (LB) was obtained from wampee seeds by extraction, separation, and structural identification It was demonstrated through cell and mouse experiments that LB has a strong hypolipidemic effect in vivo and in vitro. The results showed that: LB could effectively inhibit the activities of α-amylase, α-glucosidase, and pancreatic lipase, with IC50 values of 18.43, 15.27, and 0.16 mg/mL, respectively. Acting on oleic acid (OA)-induced high-fat HepG2 cells, when the LB dose was 5 μg/mL, triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) contents decreased by 30.77%, 30.00%, 58.33%, 39.97%, 38.84%, and 43.90%, respectively. However, high-density lipoprotein cholesterol (HDL-C), catalase (CAT), and low-density lipoprotein receptor (LDLR) contents increased by 83.33%, 253.37%, and 60.97%, respectively. After 6 weeks of feeding high-fat mice with LB, their body weight significantly decreased compared with that of the high-fat control group (P<0.05). When a 60 mg/kg LB dose was provided, TC, TG, and LDL-C contents decreased by 29.18%, 23.69%, and 70.27%, respectively, and HDL-C content increased by 50.51%. The results showed that the four indices of body weight and blood lipids of high-fat mice were all well-controlled. These results suggest that LB is effective at reducing blood lipids in cells and in vivo, and is a potential dietary hypolipidemic compound.