乳粉中主要B族维生素检测的GB和AOAC方法比较分析

Comparative Study on GB and AOAC Methods to Determine Primary B Vitamins in Milk Powder

摘要:

为探究维生素B1、B2、B3（烟酸）、B6的国家标准方法和AOAC官方方法检测结果的可比性和误差来源，该研究通过对测试结果的F检验、显著性差异检验对维生素B1、B2、B3、B6的国家标准方法和AOAC官方方法进行了等效分析、并通过双向单侧t检验（TOST）对维生素B2进行了详尽的比较分析。分析结果表明，维生素B1、维生素B3和维生素B6国家标准方法和AOAC官方方法之间无显著性差异，而维生素B2国家标准方法和AOAC方法之间机理存在差异，通过等效性分析后数据结果表明两种方法等效不好；实验中发现在乳粉基质中添加酸性磷酸酶能够获得更接近可比性数据结果。

Abstract:

The equivalence between the national standard and official AOAC methods for determining vitamins B1, B2, B3 (niacin), and B6 in milk powder was analyzed by performing the F-test and significance test on the obtained measurements to explore the comparability and error sources between these methods. Furthermore, vitamin B2 results were compared and analyzed in detail by a two-way one-sided t-test (TOST). The results indicated no significant difference between the national standard and AOAC official methods in determining vitamins B1, B3, and B6. However, for vitamin B2, a difference was observed between the two methods in terms of their mechanisms. The equivalence analysis results showed that the two methods are not effectively equivalent. Addition of acid phosphatase to the milk powder matrix was found to help obtain comparable results.