辣木叶水提物对H2O2致HepG2细胞氧化损伤的保护作用

Protective Effects of an Aqueous Extract of Moringa oleifera Leaves against H2O2-Induced Oxidative Damage in HepG2 Cells

摘要:

该研究从细胞水平对辣木叶水提物的抗氧化活性进行研究。以过氧化氢（hydrogen peroxide，H2O2）诱导氧化损伤的人体肝癌细胞（Human hepatoma cell，HepG2 cell）为模型，加入不同浓度的辣木叶水提物进行保护干预，利用MTT法测定细胞存活率，同时测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）等抗氧化活性指标，探究辣木叶水提物对H2O2诱导氧化损伤细胞的保护作用。结果表明，辣木叶水提物可明显提高经H2O2诱导损伤的HepG2细胞存活率（p<0.05），其中高浓度辣木叶水提物（500和600 μg/mL）可使细胞存活率恢复到将近100%。此外，与模型组相比，不同剂量辣木叶水提物可明显提高HepG2细胞的GSH-Px、SOD活性水平（p<0.05）和降低细胞的MDA含量（p<0.05），其中GSH-Px含量提高了20.77%~151.74%，SOD活力提高了22.24%~139.07%，MDA含量下降了12.51%~34.04%，且其变化程度具有剂量依赖效应。综上所述，辣木叶水提物在细胞水平具有一定的抗氧化活性，研究结果可为辣木叶抗氧化功能产品的开发提供参考依据。

Abstract:

The antioxidant activity of anaqueous extract of Moringa oleifera leaves was investigated at the cellular level. Taking human hepatoma cells (HepG2 cells) injured by hydrogen peroxide (H2O2)-induced oxidative stress as the model, and the administration with different concentrations of the aqueous extract of Moringa oleifera leaves was for protective intervention. MTT method was used to measure the cell survival rate, and the antioxidant activity indexes such as levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) were determined, to evaluate the protective effect of the aqueous extract of Moringa oleifera leaves against H2O2-induced oxidative damage in cells. The results showed that the survival rate of the HepG2 cells injured by H2O2 increased significantly (p<0.05) by the aqueous extract of Moringa oleifera leaves, with the cell survival rate recovered almost 100% athigh concentrations of the aqueous Moringa oleifera leaf extract (500 and 600 μg/mL). In addition, compared with the model group, the administration of the aqueous extract of Moringa oleifera leaves increased significantly (p<0.05) the activities of GSH-Px and SOD and decreased significantly (p<0.05) the MDA contents in a dose-dependent manner: increase by 20.77%~151.74% for GSH-Px, increase by 22.24%~139.07% for SOD, and decrease by 12.51%~34.04% for MDA. In summary, the aqueous extract of Moringa oleifera leaves has certain antioxidant activity at the cellular level, which can provide a reference for the development of antioxidant products of Moringa oleifera leaves.