Abstract:In this study, the effects of Ganoderma lucidum polysaccharide on the proliferation and apoptosis of human lung cancer A549 cells were investigated. Lung cancer A549 cells were divided into a blank group, drug control group, and low-, medium- and high-concentration groups. The cells in each group were intervened to detect the changes in apoptosis and proliferation ability, as well as the expression of apoptotic proteins Bcl-2, PI3K and Akt. The results showed that, compared with other groups, the high-concentration group had a lower cell proliferation rate, and its cell proliferation rate decreased from 44.37% to 20.11% when the time of intervention with Ganoderma lucidum polysaccharide was extended. Compared with other groups, the high-concentration group had a higher apoptosis rate, and its apoptosis rate increased from 46.52% to 77.93% when the time of intervention with Ganoderma lucidum polysaccharide was extended. Compared with other groups, the high-concentration group had lower relative expression levels of Bcl-2, PI3K and Akt. The intervention with Ganoderma lucidum polysaccharide can inhibit the proliferation of lung cancer A549 cells and promote the apoptosis of lung cancer A549 cells. Its ability has nothing to do with its own cytotoxicity, but may be related to the regulation of the expression of apoptosis-related proteins, Bcl-2, PI3K and Akt. These results indicate that Ganoderma lucidum polysaccharide can inhibit the development and spread of lung cancer A549 cells and regulate the proliferation and apoptosis of human lung cancer A549 cells.
