地龙蛋白胶囊对大鼠动静脉血栓生成的抑制作用

Inhibitory Effect of Earthworm Protein Capsules on Arteriovenous Thrombosis in Rats

摘要:

通过对小鼠的凝、出血时间，动、静脉血栓造模实验以及对大鼠凝血三项的检测实验，验证活性地龙蛋白胶囊在动物体内抗凝及抑制血栓生成的活性。ICR小鼠随机分为空白对照组，阳性药组，地龙低（22.50 mg/kg）、中（45.00 mg/kg）、高剂量组（90.00 mg/kg），每天给予灌胃给药（1 mL/100 g），连续7 d。末次给药后1 h，于小鼠尾尖剪断和内眦球后静脉丛取血分别观察小鼠出、凝血时间。另有SD大鼠随机分为假手术组、模型组、阳性药组（13.00 mg/kg）、地龙低（15.63 mg/kg）、中（31.26 mg/kg）、高剂量组（62.52 mg/kg）。每天给予灌胃给药（1 mL/100 g），连续7 d。末次给药1 h后计算两侧颈总动脉及下腔静脉血栓形成抑制率以及眼底静脉丛取血观察凝血酶原时间（PT）、鞣花酸活化部分凝血活酶时间（APTT）、凝血酶时间（TT）。与正常组比较，地龙胶囊可以延长小鼠尾出血及凝血时间（p<0.01），且具有剂量依赖性，高剂量组（90.00 mg/kg）延长尾出血活性（18.88 min）及凝血效果（82.63 s）最好。另外，与模型组相比，地龙胶囊随剂量升高抑栓效果逐渐增强，高剂量组（62.52 mg/kg）活性最好（湿重抑制率76.53%；干重抑制率72.49%）。实验表明地龙胶囊具有抑制血栓生成的作用。

Abstract:

The pharmacological activity of active earthworm protein capsules (AEPC) in animals was verified by three tests on the coagulation and blood time of mice, arterial and venous thrombosis modeling experiments and rat coagulation tests. ICR mice were randomly divided into blank control group, positive group, low-dose (22.50 mg/kg), medium-dose (45.00 mg/kg), high-dose group (90.00 mg/kg). After grouping, mice were given intragastric administration (1 mL/100 g) every day for 7 consecutive days. One hour after the last administration, blood samples were taken from the tail tip and the retrobulbar vein plexus to observe the bleeding and coagulation time. SD rats were randomly divided into 6 groups, namely sham operation group, model group, positive group (13.00 mg/kg), low-dose (15.63 mg/kg), medium-dose (31.26 mg/kg), high-dose group (62.52 mg/kg), 11 in each group. After grouping, daily intragastric administration (1 mL/100 g) was given for 7 consecutive days. One hour after the last administration, the inhibition rates of thrombosis in both common carotid arteries and inferior vena cava were calculated, and the blood samples from ocular fundus venous plexus were taken to observe prothrombin time (PT), ellagic acid activated partial thromboplastin time (APTT), thrombin time (TT), 8 animals were taken from each group. Compared with the normal group, AEPC can prolong the tail bleeding and coagulation time of mice (p<0.01), and it is dose dependent. The high-dose group (90.00 mg/kg) had the best prolonged tail bleeding activity (18.88 min) and coagulation effect (82.63 min). In addition, compared with the model group, the effect of AEPC on thrombosis was gradually increased with the increase of dose. The high-dose group (62.52 mg/kg) had the best activity (wet weight inhibition rate 76.53; dry weight inhibition rate 72.49). The experimental data show that AEPC has a significant effect on the inhibition of thrombosis.